Reporter

Part:BBa_K1523101:Design

Designed by: Haotian Wang   Group: iGEM14_LZU-China   (2014-09-11)


PyeaR->Riboflavin synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We found that the part BBa_K381001 by iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and nitrite. So we perfomed an experience by this part to see if this part can detect PNP(p-Nitrophenol).

We got a good result.You can see the PNP can also induce the green fluorescence.

So we composite the K381001‘s promoter and RBS with a coding sequence of riboflavin(K1172303,iGEM13_Bielefeld-Germany)

Source

By registry

References